|
KMID : 0370219980420010082
|
|
Yakhak Hoeji
1998 Volume.42 No. 1 p.82 ~ p.88
|
|
|
Effect of Hepatoprotective Agents and Bile Acids on TNF-α Production in Macrophage Cell Lines
|
|
조재열/Cho JY
박지수/유은숙/백경업/박명환/Park JS/Yoo ES/Baik KU/Park MH
|
|
|
Abstract
|
|
|
|
The effect of hepatoprotective agents and bile acids on tumor necrosis factor-alpha, (TNF-alpha) production in murine and human macrophage cell line (RAW264.7 and U937) was inve stigated. The hepatoprotective agents including silymarin and its major component, silybin, significantly inhibited TNF-alpha production in a concentration dependent manner (IC50 of silybin=67.7mcg/ml (140.3mcM)). In differentiated U937 cells, especially, silybin showed more effective inbitory activity (IC50=35.1mcg/ml (72.7mcM)). These results suggest that silymarin and silybin may inhibit TNF-alpha production in the process of hepatic diseases in human. However, biphenyldimethyl dicarboxylate (DDB) was not effective. In the case of bile acids, chenodeoxycholic acid (CDCA) showed a concentration dependent inhibitory effect on TNF-alpha production (IC50 of CDCA= 71.5mcg/ml (182.1mcM)). In contrast, glycine or taurine conjugated form (G-CDCA or T-CDCA) restored to the control level or significantly increased TNF-alpha production. And also ursodeoxycholic acid (UDCA) and its conjugated forms (G-UDCA and T-UDCA) showed a variety of patterns on TNF-alpha production by changes of functional groups and concentration. These results also indicate that bile acids may regulate TNF-alpha production in normal hepatic function or disease conditions.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|